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DNA-magnetic bead detection using disposable cards and the anisotropic magnetoresistive sensor

Thi Hien Le and Khanh Quynh Le and Thi Huyen Vu and Dinh Tu Bui and Phuong DM and Nhung PH and Thi Huong Giang Do and Huu Duc Nguyen (2016) DNA-magnetic bead detection using disposable cards and the anisotropic magnetoresistive sensor. Advances in Natural Sciences: Nanoscience and Nanotechnology, 7 . 045006. ISSN 2043-6262

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Official URL: http://dx.doi.org/10.1088/2043-6262/7/4/045006

Abstract

A disposable card incorporating specific DNA probes targeting the 16 S rRNA gene of Streptococcus suis was developed for magnetically labeled target DNA detection. A singlestranded target DNA was hybridized with the DNA probe on the SPA/APTES/PDMS/Si asprepared card, which was subsequently magnetically labeled with superparamagnetic beads for detection using an anisotropic magnetoresistive (AMR) sensor. An almost linear response between the output signal of the AMR sensor and amount of single-stranded target DNA varied from 4.5 to 18 pmol was identified. From the sensor output signal response towards the mass of magnetic beads which were directly immobilized on the disposable card surface, the limit of detection was estimated about 312 ng ferrites, which corresponds to 3.8 μemu. In comparison with DNA detection by conventional biosensor based on magnetic bead labeling, disposable cards are featured with higher efficiency and performances, ease of use and less running cost with respects to consumables for biosensor in biomedical analysis systems operating with immobilized bioreceptor

Item Type:Article
Subjects:Engineering Physics
ISI/Scopus-indexed journals
Divisions:Faculty of Engineering Physics and Nanotechnology (FEPN)
ID Code:2268
Deposited By: Dr Giang Do
Deposited On:27 Dec 2016 07:27
Last Modified:27 Dec 2016 07:27

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