TY  - JOUR
ID  - SisLab2268
UR  - http://dx.doi.org/10.1088/2043-6262/7/4/045006
A1  - Le, Thi Hien
A1  - Le, Khanh Quynh
A1  - Vu, Thi Huyen
A1  - Bui, Dinh Tu
A1  - DM, Phuong
A1  - PH, Nhung
A1  - Do, Thi Huong Giang
A1  - Nguyen, Huu Duc
Y1  - 2016/08/24/
N2  - A disposable card incorporating specific DNA probes targeting the 16 S rRNA gene of
Streptococcus suis was developed for magnetically labeled target DNA detection. A singlestranded
target DNA was hybridized with the DNA probe on the SPA/APTES/PDMS/Si asprepared
card, which was subsequently magnetically labeled with superparamagnetic beads for
detection using an anisotropic magnetoresistive (AMR) sensor. An almost linear response
between the output signal of the AMR sensor and amount of single-stranded target DNA varied
from 4.5 to 18 pmol was identified. From the sensor output signal response towards the mass of magnetic beads which were directly immobilized on the disposable card surface, the limit of detection was estimated about 312 ng ferrites, which corresponds to 3.8 ?emu. In comparison with DNA detection by conventional biosensor based on magnetic bead labeling, disposable cards are featured with higher efficiency and performances, ease of use and less running cost with respects to consumables for biosensor in biomedical analysis systems operating with immobilized bioreceptor
PB  - IOP Publishing
JF  - Advances in Natural Sciences: Nanoscience and Nanotechnology
VL  - 7
SN  - 2043-6262
TI  - DNA-magnetic bead detection using disposable cards and the anisotropic magnetoresistive sensor
AV  - public
ER  -