eprintid: 2268
rev_number: 11
eprint_status: archive
userid: 16
dir: disk0/00/00/22/68
datestamp: 2016-12-27 07:27:52
lastmod: 2016-12-27 07:27:52
status_changed: 2016-12-27 07:27:52
type: article
metadata_visibility: show
creators_name: Le, Thi Hien
creators_name: Le, Khanh Quynh
creators_name: Vu, Thi Huyen
creators_name: Bui, Dinh Tu
creators_name: DM, Phuong
creators_name: PH, Nhung
creators_name: Do, Thi Huong Giang
creators_name: Nguyen, Huu Duc
creators_id: lehien@vnu.edu.vn
creators_id: buidinhtu@vnu.edu.vn
creators_id: giangdth@vnu.edu.vn
creators_id: ducnh@vnu.edu.vn
title: DNA-magnetic bead detection using disposable cards and the anisotropic magnetoresistive sensor
ispublished: pub
subjects: Phys
subjects: isi
divisions: fac_physic
abstract: A disposable card incorporating specific DNA probes targeting the 16 S rRNA gene of
Streptococcus suis was developed for magnetically labeled target DNA detection. A singlestranded
target DNA was hybridized with the DNA probe on the SPA/APTES/PDMS/Si asprepared
card, which was subsequently magnetically labeled with superparamagnetic beads for
detection using an anisotropic magnetoresistive (AMR) sensor. An almost linear response
between the output signal of the AMR sensor and amount of single-stranded target DNA varied
from 4.5 to 18 pmol was identified. From the sensor output signal response towards the mass of magnetic beads which were directly immobilized on the disposable card surface, the limit of detection was estimated about 312 ng ferrites, which corresponds to 3.8 μemu. In comparison with DNA detection by conventional biosensor based on magnetic bead labeling, disposable cards are featured with higher efficiency and performances, ease of use and less running cost with respects to consumables for biosensor in biomedical analysis systems operating with immobilized bioreceptor
date: 2016-08-24
date_type: published
publisher: IOP Publishing
official_url: http://dx.doi.org/10.1088/2043-6262/7/4/045006
full_text_status: public
publication: Advances in Natural Sciences: Nanoscience and Nanotechnology
volume: 7
pagerange: 045006
refereed: TRUE
issn: 2043-6262
funders: Vietnam National University, Hanoi (VNU)
projects: QGTD 13.24
projects: QG.16.26
citation:   Le, Thi Hien and Le, Khanh Quynh and Vu, Thi Huyen and Bui, Dinh Tu and DM, Phuong and PH, Nhung and Do, Thi Huong Giang and Nguyen, Huu Duc  (2016) DNA-magnetic bead detection using disposable cards and the anisotropic magnetoresistive sensor.  Advances in Natural Sciences: Nanoscience and Nanotechnology, 7 .   045006.  ISSN 2043-6262     
document_url: https://eprints.uet.vnu.edu.vn/eprints/id/eprint/2268/1/Hien_2016_Adv._Nat._Sci%253A_Nanosci._Nanotechnol._7_045006.pdf