eprintid: 3307
rev_number: 7
eprint_status: archive
userid: 270
dir: disk0/00/00/33/07
datestamp: 2019-12-09 03:31:02
lastmod: 2019-12-09 03:31:02
status_changed: 2019-12-09 03:31:02
type: article
metadata_visibility: show
creators_name: Do, Loc Quang
creators_name: Tran, Thi Thuy Ha
creators_name: Bui, Thanh Tung
creators_name: Dau, Van Thanh
creators_name: Nguyen, Ngoc Viet
creators_name: Chu, Duc Trinh
creators_name: Jen, Chun-Ping
creators_id: locdq@hus.edu.vn
creators_id: tttha@gmail.com
creators_id: tungbt@vnu.edu.vn
creators_id: dauv@sc.sumitomo-chem.co.jp
creators_id: vietnn.mt@gmail.com
creators_id: trinhcd@vnu.edu.vn
creators_id: Chun-Ping@gmail.com
title: Dielectrophoresis Microfluidic Enrichment Platform with Built-In Capacitive Sensor for Rare Tumor Cell Detection
ispublished: pub
subjects: isi
divisions: cetr_uet
divisions: fac_fet
abstract: The manipulation and detection of rare cells are important for many applications in early disease diagnosis and medicine. This study presents a dielectrophoresis (DEP) microfluidic enrichment platform combined with a built-in capacitive sensor for circulating tumor cell detection. The microchip is composed of a lollipop-shaped gold microelectrode structure under a polydimethylsiloxane chamber. A prototype of the device was fabricated using standard micromachining technology. With the proposed device, target cells (in this study, A549 non-small human lung carcinoma and S-180 sarcoma cell lines) are firstly guided toward the center of the working chamber via DEP forces. Then, the target cells are captured by an electrode immobilized by anti-EGFR, which has high affinity toward the target cells. After the cell concentration process, the differential capacitance is read to detect the presence of the target cells. Numerical simulations and measurement experiments were performed to demonstrate the high sensitivity of differential capacitive sensing. The obtained results show high sensitivity for S-180 cell detection (3 mV/cell). The proposed platform is suitable for rapid cancer diagnoses and other metabolic disease applications.
date: 2018-06
date_type: published
official_url: http://doi.org/10.1007/s13206-017-2204-x
id_number: doi:10.1007/s13206-017-2204-x
full_text_status: none
publication: BioChip Journal
volume: 12
number: 2
pagerange: 114-122
refereed: TRUE
issn: 1976-0280
citation:   Do, Loc Quang and Tran, Thi Thuy Ha and Bui, Thanh Tung and Dau, Van Thanh and Nguyen, Ngoc Viet and Chu, Duc Trinh and Jen, Chun-Ping  (2018) Dielectrophoresis Microfluidic Enrichment Platform with Built-In Capacitive Sensor for Rare Tumor Cell Detection.  BioChip Journal, 12  (2).   pp. 114-122.  ISSN 1976-0280