relation: https://eprints.uet.vnu.edu.vn/eprints/id/eprint/4591/
title: IN VITRO SELECTION OF Chrysanthemum × morifolium FOR SALT TOLERANCE
creator: La, Viet Hong
creator: Le, Hoang Duc
creator: Chu, Duc Ha
creator: Cao, Phi Bang
creator: Phung, Thi Ha
subject: Agriculture Technology
description: The chrysanthemum is one of the most widely cultivated plants and also one of the most important ornamental plants in the world. In this study, we established a systematic regeneration of directly adventitious shoots from the leaf segments of chrysanthemum, then determined the concentration of NaCl as a direct selective factor for the adventitious   shoots, and finally analyzed the genetic diversity of some selected clones by ISSR-PCR analysis. The results showed that the most suitable medium for the adventitious shoot regeneration from leaf explants in chrysanthemum was MS, 30 g/l sucrose, 7 g/l agar, pH 5.8, supplemented with 0.5 mg/l BAP. After 5 cultured weeks, the number of shoots per explants, the number of leaves per shoot, and the length of the shoot were 4.37 (shoots/explant), 4.25 (amount of leaves/shoot), and 1.96 (cm), respectively. The medium for direct selection of salinity�tolerant in vitro shoots was the adventitious shoot regeneration medium supplemented with 100 mM NaCl. The 873 primer [5 '- (GACA)4 -3'] was suitable for analyzing the genetic relationships of selected chrysanthemum lines. By ISSR-PCR analysis, the following five selective chrysanthemum lines (D1-D5) showed the genetic diversity. These chrysanthemum lines are potential materials for more analysis of salinity tolerance
date: 2021-07
type: Article
type: PeerReviewed
identifier:   La, Viet Hong and Le, Hoang Duc and Chu, Duc Ha and Cao, Phi Bang and Phung, Thi Ha  (2021) IN VITRO SELECTION OF Chrysanthemum × morifolium FOR SALT TOLERANCE.  TNU Journal of Science and Technology, 226  (10).   pp. 316-322.