eprintid: 4591
rev_number: 6
eprint_status: archive
userid: 465
dir: disk0/00/00/45/91
datestamp: 2021-08-11 01:43:37
lastmod: 2021-08-11 01:43:37
status_changed: 2021-08-11 01:43:37
type: article
metadata_visibility: show
creators_name: La, Viet Hong
creators_name: Le, Hoang Duc
creators_name: Chu, Duc Ha
creators_name: Cao, Phi Bang
creators_name: Phung, Thi Ha
creators_id: laviethong.sp2@gmail.com
creators_id: cd.ha@vnu.edu.vn
title: IN VITRO SELECTION OF Chrysanthemum × morifolium FOR SALT TOLERANCE
ispublished: pub
subjects: at
divisions: fat_uet
abstract: The chrysanthemum is one of the most widely cultivated plants and also one of the most important ornamental plants in the world. In this study, we established a systematic regeneration of directly adventitious shoots from the leaf segments of chrysanthemum, then determined the concentration of NaCl as a direct selective factor for the adventitious 
shoots, and finally analyzed the genetic diversity of some selected clones by ISSR-PCR analysis. The results showed that the most suitable medium for the adventitious shoot regeneration from leaf explants in chrysanthemum was MS, 30 g/l sucrose, 7 g/l agar, pH 5.8, supplemented with 0.5 mg/l BAP. After 5 cultured weeks, the number of shoots per explants, the number of leaves per shoot, and the length of the shoot were 4.37 (shoots/explant), 4.25 (amount of leaves/shoot), and 1.96 (cm), respectively. The medium for direct selection of salinity�tolerant in vitro shoots was the adventitious shoot regeneration medium supplemented with 100 mM NaCl. The 873 primer [5 '- (GACA)4 -3'] was suitable for analyzing the genetic relationships of selected chrysanthemum lines. By ISSR-PCR analysis, the following five selective chrysanthemum lines (D1-D5) showed the genetic diversity. These chrysanthemum lines are potential materials for more analysis of salinity tolerance
date: 2021-07
date_type: published
full_text_status: none
publication: TNU Journal of Science and Technology
volume: 226
number: 10
pagerange: 316-322
refereed: TRUE
related_url_url: https://doi.org/10.34238/tnu-jst.4445
citation:   La, Viet Hong and Le, Hoang Duc and Chu, Duc Ha and Cao, Phi Bang and Phung, Thi Ha  (2021) IN VITRO SELECTION OF Chrysanthemum × morifolium FOR SALT TOLERANCE.  TNU Journal of Science and Technology, 226  (10).   pp. 316-322.