%0 Conference Paper
%A Bui, Thi Thanh Hien
%A Nguyen, Thi Yen Chi
%A Chu, Duc Ha
%A Bui, Van Hung
%A Ngo, Thi Hong Tuoi
%A Nguyen, Quoc Trung
%A Tran, Van Quang
%B Hội nghị Công nghệ Sinh học Toàn quốc năm 2021
%C Thái Nguyên
%F SisLab:4639
%T DNA SEQUENCE POLYMORPHISM OF Rc GENE IN 'YEN BAI' LOCAL   PURPLE STICKY RICE
%U https://eprints.uet.vnu.edu.vn/eprints/id/eprint/4639/
%X Rc is one of the most important regulatory genes for proanthocyanidin accumulation in the rice pericarp. A study   on mutation and sequence polymorphism of the Rc gene will provide evidence for understanding the evolution of   rice cultivars. The aim of this study was to archive a full-length genomic DNA sequence and analyze   polymorphism in the Rc gene of Nepcam YB rice, a local pigmented rice in Yen Bai province. By using 18   primer pairs, full-length sequence with down-stream and up-stream of the Rc gene of NepCam YB variety was   successfully assembled and aligned by BioEdit software with 7500 bp. We found that the Rc gene from NepCam   YB variety contains 8 introns and 9 exons. Sequence polymorphism analysis has revealed 14 bp deletion in the   fifth introns and 54 SNPs of Rc gene was detected by comparing with 8 representative rice cultivars. Next, our   phylogeny analysis revealed that Nepcam YB was closely related to the Japonica Jefferson cultivar. In this  study, full-length sequence of the Rc gene in red pericarp Nepcam YB was successfully archived and is   preliminary data for understanding domestication of Vietnamese local rice.