eprintid: 4639
rev_number: 9
eprint_status: archive
userid: 465
dir: disk0/00/00/46/39
datestamp: 2021-11-27 02:28:34
lastmod: 2021-11-27 02:28:34
status_changed: 2021-11-27 02:28:34
type: conference_item
metadata_visibility: show
creators_name: Bui, Thi Thanh Hien
creators_name: Nguyen, Thi Yen Chi
creators_name: Chu, Duc Ha
creators_name: Bui, Van Hung
creators_name: Ngo, Thi Hong Tuoi
creators_name: Nguyen, Quoc Trung
creators_name: Tran, Van Quang
creators_id: cd.ha@vnu.edu.vn
title: DNA SEQUENCE POLYMORPHISM OF Rc GENE IN 'YEN BAI' LOCAL 
PURPLE STICKY RICE
ispublished: pub
subjects: at
divisions: fat_uet
abstract: Rc is one of the most important regulatory genes for proanthocyanidin accumulation in the rice pericarp. A study 
on mutation and sequence polymorphism of the Rc gene will provide evidence for understanding the evolution of 
rice cultivars. The aim of this study was to archive a full-length genomic DNA sequence and analyze 
polymorphism in the Rc gene of Nepcam YB rice, a local pigmented rice in Yen Bai province. By using 18 
primer pairs, full-length sequence with down-stream and up-stream of the Rc gene of NepCam YB variety was 
successfully assembled and aligned by BioEdit software with 7500 bp. We found that the Rc gene from NepCam 
YB variety contains 8 introns and 9 exons. Sequence polymorphism analysis has revealed 14 bp deletion in the 
fifth introns and 54 SNPs of Rc gene was detected by comparing with 8 representative rice cultivars. Next, our 
phylogeny analysis revealed that Nepcam YB was closely related to the Japonica Jefferson cultivar. In this
study, full-length sequence of the Rc gene in red pericarp Nepcam YB was successfully archived and is 
preliminary data for understanding domestication of Vietnamese local rice.
full_text_status: public
pres_type: paper
event_title: Hội nghị Công nghệ Sinh học Toàn quốc năm 2021
event_location: Thái Nguyên
event_type: conference
refereed: TRUE
citation:   Bui, Thi Thanh Hien and Nguyen, Thi Yen Chi and Chu, Duc Ha and Bui, Van Hung and Ngo, Thi Hong Tuoi and Nguyen, Quoc Trung and Tran, Van Quang   DNA SEQUENCE POLYMORPHISM OF Rc GENE IN 'YEN BAI' LOCAL PURPLE STICKY RICE.  In: Hội nghị Công nghệ Sinh học Toàn quốc năm 2021, Thái Nguyên.     
document_url: https://eprints.uet.vnu.edu.vn/eprints/id/eprint/4639/1/2021.21%20%5BHoi%20nghi%20CNSH%20Toan%20quoc%5D%20Rc%20gene.pdf