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La, Viet Hong and Le, Hoang Duc and Chu, Duc Ha and Cao, Phi Bang and Phung, Thi Ha (2021) IN VITRO SELECTION OF Chrysanthemum × morifolium FOR SALT TOLERANCE. TNU Journal of Science and Technology, 226 (10). pp. 316-322.

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The chrysanthemum is one of the most widely cultivated plants and also one of the most important ornamental plants in the world. In this study, we established a systematic regeneration of directly adventitious shoots from the leaf segments of chrysanthemum, then determined the concentration of NaCl as a direct selective factor for the adventitious shoots, and finally analyzed the genetic diversity of some selected clones by ISSR-PCR analysis. The results showed that the most suitable medium for the adventitious shoot regeneration from leaf explants in chrysanthemum was MS, 30 g/l sucrose, 7 g/l agar, pH 5.8, supplemented with 0.5 mg/l BAP. After 5 cultured weeks, the number of shoots per explants, the number of leaves per shoot, and the length of the shoot were 4.37 (shoots/explant), 4.25 (amount of leaves/shoot), and 1.96 (cm), respectively. The medium for direct selection of salinity�tolerant in vitro shoots was the adventitious shoot regeneration medium supplemented with 100 mM NaCl. The 873 primer [5 '- (GACA)4 -3'] was suitable for analyzing the genetic relationships of selected chrysanthemum lines. By ISSR-PCR analysis, the following five selective chrysanthemum lines (D1-D5) showed the genetic diversity. These chrysanthemum lines are potential materials for more analysis of salinity tolerance

Item Type: Article
Subjects: Agriculture Technology
Divisions: Faculty of Agriculture Technology (FAT)
Depositing User: Ph.D. Ha Chu
Date Deposited: 11 Aug 2021 01:43
Last Modified: 11 Aug 2021 01:43

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