Bui, Thi Thanh Hien and Nguyen, Thi Yen Chi and Chu, Duc Ha and Bui, Van Hung and Ngo, Thi Hong Tuoi and Nguyen, Quoc Trung and Tran, Van Quang
DNA SEQUENCE POLYMORPHISM OF Rc GENE IN 'YEN BAI' LOCAL
PURPLE STICKY RICE.
In: Hội nghị Công nghệ Sinh học Toàn quốc năm 2021, Thái Nguyên.
Abstract
Rc is one of the most important regulatory genes for proanthocyanidin accumulation in the rice pericarp. A study
on mutation and sequence polymorphism of the Rc gene will provide evidence for understanding the evolution of
rice cultivars. The aim of this study was to archive a full-length genomic DNA sequence and analyze
polymorphism in the Rc gene of Nepcam YB rice, a local pigmented rice in Yen Bai province. By using 18
primer pairs, full-length sequence with down-stream and up-stream of the Rc gene of NepCam YB variety was
successfully assembled and aligned by BioEdit software with 7500 bp. We found that the Rc gene from NepCam
YB variety contains 8 introns and 9 exons. Sequence polymorphism analysis has revealed 14 bp deletion in the
fifth introns and 54 SNPs of Rc gene was detected by comparing with 8 representative rice cultivars. Next, our
phylogeny analysis revealed that Nepcam YB was closely related to the Japonica Jefferson cultivar. In this
study, full-length sequence of the Rc gene in red pericarp Nepcam YB was successfully archived and is
preliminary data for understanding domestication of Vietnamese local rice.
Actions (login required)
 |
View Item |
