Bui, Thi Thu Huong and Nguyen, Thi Hong and Chu, Duc Ha and Ha, Thi Quyen and Pham, Phuong Thu and Phung, Thi Thu Huong and Le, Thi Ngoc Quynh and Nguyen, Quoc Trung and Dong, Huy Gioi and Nguyen, Thanh Hai and Ninh, Thi Thao
(2022)
Recent Advances and Future Perspectives for the Improvement
of Stress Tolerance in Rice Breeding Using CRISPR/Cas9.
Tạp chí Khoa học Nông nghiệp Việt Nam
.
pp. 123-132.
Abstract
Rice (Oryza sativa L.) is one of the most important staple crops that is widely cultivated in the world. Due to the
critical role of rice in the global food security, great efforts have been made in order to develop new rice varieties with
good agronomic traits, such as biotic and abiotic stress tolerance. The CRISPR/Cas9 has emerged as a promising
system for the improvement of various traits of crop plants because of its efficiency, simplicity, and versatility. In this
mini review, we discussed the applications of the CRISPR/Cas9 gene editing system to improve a wide range of traits
in rice varieties adapted to unfavorable conditions. Specifically, a number of functional and regulatory genes that are
associated with diseases (rice blast, bacterial blight) and pesticide resistance and abiotic stress (salinity, drought, and
cold) tolerance have been functionally characterized via the mutants produced by the CRISPR/Cas9 system.
Additionally, the advances and limitations of using CRISPR/Cas9 system in rice plants were discussed. Taken
together, our paper could provide a solid foundation for further application of genome editing tools in plant breeding
for tackling climate change.
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